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TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis

Amelie Fassbender, Peter Simsa, Cleophas M Kyama, Etienne Waelkens, Attila Mihalyi, Christel Meuleman, Olivier Gevaert, Raf Van de Plas, Bart de Moor and Thomas M D'Hooghe1234*

Author Affiliations

1 Leuven University Fertility Centre, Department of Obstetrics & Gynaecology, University Hospital Gasthuisberg, Leuven, Belgium

2 Division of Reproductive Health and Biology, Institute of Primate Research, P.O. Box 24481-00502 Karen, Nairobi, Kenya

3 Biochemistry Section, Department of Molecular Cell Biology, Campus Gasthuisberg, Leuven, Belgium

4 Department of Electrical Engineering, ESAT-SCD, K.U.Leuven, Kasteelpark-Arenberg 10, B-3001 Heverlee, Belgium

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Reproductive Biology and Endocrinology 2011, 9:44  doi:10.1186/1477-7827-9-44

The electronic version of this article is the complete one and can be found online at:

Received:29 March 2011
Accepted:6 April 2011
Published:6 April 2011

© 2011 Fassbender et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Since publication of our article [1], we have realised that we did not include the full data in Table 1 and missed the word (TOF) in the result/discussion section. We have provided here the adapted sentence and another version of the table, including all the information intended.

Table 1. The representative molecular weights of the proteins identified in the mRNA Microarray study [Thirteen] [2]


"Therefore, we plan to repeat this study in a larger sample size including well defined endometrial samples obtained during menstrual, follicular and secretory phase, to validate the reproducibility of SELDI-TOF MS technology in these samples and to identify the protein peaks observed after proteomic analysis, which are expensive and labour intense requiring High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) and matrix assisted laser desorption ionization Time-of-Flight-Mass Spectrometry (MALDI-TOF/TOF MS)."


  1. Fassbender Amelie, Simsa Peter, Kyama CleophasM, Waelkens Etienne, Mihalyi Attila, Meuleman Christel, Gevaert Olivier, Van de Plas Raf, de Moor Bart, D'Hooghe ThomasM: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis.

    Reproductive Biology and Endocrinology 2010, 8:123. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  2. Sherwin JR, Sharkey AM, Mihalyi A, Simsa P, Catalano RD, D'Hooghe TM: Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis.

    Hum Reprod 2008, 23(5):1063-1068. PubMed Abstract | Publisher Full Text OpenURL