Figure 4.

Localization of BMP15 and GDF9 mRNA in antral follicles. BMP15 (A-D) and GDF9 (E-H) mRNA were detected using in situ hybridization. Positive cells are stained blue. The sections of calf (A, B, E, F) and adult (C, D, G, H) ovaries were individually hybridized with DIG-labeled anti-sense (A, C, E, G) and sense (negative control; B, D, F, H) RNA probes. The scale bar is 100 μm.