QPCR analysis of BMP15 and GDF9 mRNA in the cells of ovaries derived from calves or cows. Total RNA was extracted from oocytes, cumulus cells and mural granulosa cells. The expression of these mRNAs was normalized to the expression of GAPDH measured in the same RNA preparation. Results of three independent experiments are summarized and expressed as the mean ± SEM. Different letters above the bars indicate significant differences (P < 0.05).
Hosoe et al. Reproductive Biology and Endocrinology 2011 9:33 doi:10.1186/1477-7827-9-33