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Establishment of an immortalized human endometrial stromal cell line with functional responses to ovarian stimuli

Munehiro Yuhki1*, Takashi Kajitani2, Takakazu Mizuno1, Yuko Aoki1 and Tetsuo Maruyama2

Author Affiliations

1 Kamakura Research Laboratories, Chugai Pharmaceutical Co., Ltd., 200 Kajiwara, Kamakura, Kanagawa 247-8530, Japan

2 Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo 160-8582, Japan

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Reproductive Biology and Endocrinology 2011, 9:104  doi:10.1186/1477-7827-9-104

Published: 1 August 2011


Studies on the mechanisms of decidualization and endometriosis are often hampered by lack of primary endometrial cells. To facilitate in vitro studies, we established a human endometrial stromal cell line, KC02-44D, immortalized with human telomerase reverse transcriptase. Upon exposure to ovarian stimuli, KC02-44D cells showed similar cytoskeletal marker or gene expression and biochemical phenotype to primary endometrial stromal cells. KC02-44D would be useful for studies of human endometrial function and its associated pathologies.