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Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist

Vance L Trudeau*, Gustavo M Somoza, Guillermo S Natale, Bruce Pauli, Jacqui Wignall, Paula Jackman, Ken Doe and Fredrick W Schueler

Reproductive Biology and Endocrinology 2010, 8:36  doi:10.1186/1477-7827-8-36

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Change in AMPHIPLEX protocol

Vance Trudeau   (2012-09-25 17:02)  University of Ottawa email

Please contact me for details in a protocol update. I highly recommend to you use metoclopromide-HCL (Sigma). It is water soluble, and therefore you do not need DMSO. This makes handling of the solutions much easier. I am open to collaborations with accredited institutions so please feel free to contact me. Sincerely, Vance Trudeau ( or

Competing interests

I am first author on this paper.


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