Treg cell gating strategy. The characterization of the subpopulation of Treg cells (CD4+CD25+FOXP3+) within the subpopulation of T lymphocytes in eutopic endometrium during the secretory cycle phases (E), ovarian endometriosis (OE), and Fallopian tube pregnancy (EP). Left panel shows the gating strategy for CD4-positive cells (P1). Middle panel is used to set the gate on double positive CD4+CD25+ cells (P2). On the right panel the histogram representing FoxP3 staining is shown (FoxP3+).
Basta et al. Reproductive Biology and Endocrinology 2010 8:116 doi:10.1186/1477-7827-8-116