The JEG3 cells were transiently transfected with CRE-luciferase and β-galactosidase reporter constructs overnight using Fugene6 (Roche) as described under Methods. The cells were stimulated with either cAMP or media. Luciferase assay was performed to assess CRH promoter activation. Calorimetric β-galactosidase assay was performed to correct for the transfection efficiency. Luciferase activity was expressed as relative light unit (RLU). (*p < 0.01 compared to media treated cells). Each experiment was performed in triplicate or quadruplicate and repeated at least three independent times.
Uh et al. Reproductive Biology and Endocrinology 2009 7:74 doi:10.1186/1477-7827-7-74