|
Localization of PS on spermatozoa labeled by annexin V-FITC in DGC-prepared sperm |
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| PS localization |
DGCprepared sperm Noncapacitated Sperm |
(n = 29) |
DGC-prepared sperm Capacitated Sperm |
(n = 29) |
|
|
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| Total (%A-) + (%A+) |
100 |
100 |
||
| Annexin V- (%A-) |
86.7 ± 1.8 (61–98) |
83.7 ± 1.8 (55–98) |
||
| Annexin V+ (%A+) |
13.3 ± 1.8 (2–39) |
100 |
16.3 ± 1.8 (2–45)S |
100 |
| Head |
14.7 ± 2.2 (0–46) |
18.6 ± 2.0 (0–57)S |
||
| Head and Midpiece |
11.5 ± 1.9 (0–38) |
15.4 ± 1.8 (0–44)S |
||
| Midpiece |
7.7 ± 1.4 (0–27) |
7.8 ± 1.3 (0–22) NS |
||
| Tail |
4.7 ± 1.2 (0–33) |
5.1 ± 1.4 (0–33) NS |
||
| Entire spermatozoon |
61.4 ± 3.6 (25–100) |
53.1 ± 3.5 (18–89)S |
||
|
Values are mean percentages ± SEM (minimum-maximum values). NS = statistically not significant compared to noncapacitated sperm (paired t-test). S = statistically significant compared to noncapacitated sperm with p < 0.05 (paired t-test) | ||||
de Vantéry Arrighi et al. Reproductive Biology and Endocrinology 2009 7:1 doi:10.1186/1477-7827-7-1 |
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