Table 1

Primers used for real-time RT-PCR

mRNA
Primer Sequence (5'-3')
Product size (bp)
Accession Number

NPY
Forward
cgctctgcgacactacatca
157
NM_012614

Reverse
tttcatttcccatcaccaca


AgRP
Forward
tgtgggccctttattagacc
156
XM_574228

Reverse
ccatatggacccccaatgt


POMC
Forward
tccatagacgtgtggagctg
174
NM_139326

Reverse
gacgtacttccggggatttt


CART
Forward
gccctggacatctactctgc
201
NM_017110

Reverse
cactgcgcactgctctcc


HPRT
Forward
cagtcccagcgtcgtgatta
137
NM_012583

Reverse
agcaagtctttcagtcctgtc



Caminos et al. Reproductive Biology and Endocrinology 2008 6:14   doi:10.1186/1477-7827-6-14