Figure 1.

Immunohistochemical staining revealing the presence of Sema-4D in the mouse ovary at all stages: (A) un-stimulated (B) after PMSG administration, (C) 6 hours after hCG administration, and (D) 24 hours after hCG administration. Sema-4D was identified as red-brown membrane staining in ovarian sections counterstained with hematoxylin; magnification ×400. Bar graph describing the relative percentage of Sema-4D expression, representing, mean ± SEM from three independent experiments.