PCR products for the cDNAs evaluated in this study. Pannels A, B and C show photographs of agarose electrophoresis for representative PCR products of the different target sequences studied: Pannel A shows PCR products for VEGF (102 bp for VEGF 120 and 234 bp for VEGF 164) of control samples (C) and NGF treated ovaries (N); additional bands corresponding to other VEGF isoforms were also visible for some samples and thus could not be considered in this study. Pannel B shows PCR product for TGFβ1 (246 bp) of control (C) and NGF treatment (N); Pannel C shows PCR products for the constitutive gene Cyclophilin (350 bp) of control (C) and NGF treatment (N). Negative controls in each photograph correspond to the lane indicated as (-). Pannels D, E and F represent graphs of PCR reactions starting with different quantities of cDNA, indicating the linear range in which each densitometry was made in order to observe differences in each experimental condition: D) Linear range for VEGF amplification (r2 = 0.9936 for VEGF 120 and r2 = 0.9863 for VEGF 164); E) Linear range for TGFβ1 amplification (r2 = 0.9996); F) Linear range for Cyclophilin amplification (r2 = 0.9988). Each graph represents PCR products obtained from a pool of cDNA samples.
Julio-Pieper et al. Reproductive Biology and Endocrinology 2006 4:57 doi:10.1186/1477-7827-4-57