Figure 2.

Quantitative RT-PCR analysis of IAP and MuERV-L. (A) Position of primers used in the experiment. (B) Pattern of IAP and MuERV-L mRNA expression. Each lane represents the mean plus standard desviation of 3 technical replicates from 3 independent prepared samples, expressed relative to the group in which the expression was the lowest. The region used for the qRT-PCR expression analysis of these elements is indicated with a line at the 3'end of each element as shown in Fig 3A. BL, blastocyst. (C) Quantitative RT-PCR of sense and antisense RNA strands from IAP and MuERV-L. Each lane represents the results of 3 replicates from 3 independent prepared samples, expressed relative to the group in which the expression was the lowest. a, b: refers to significant differences in relative transcript abundance between column (P < 0.05).