Figure 3.

Sequence of microphotograph of ES cells transformed with 5 k-mTert-EGFP during differentiation induced by LIF removal. (A) ES cell expressing EGFP; (B) three days after LIF removal the ES cells form embryonic bodies (EBs); (C) Differentiation of EBs showing a diminishes on fluorescence intensity; and (D) next stage of differentiation showing a loss of fluorescence; in some wells groups of EGFP expressing cells remain immersed into differentiated colonies.

Pericuesta et al. Reproductive Biology and Endocrinology 2006 4:5   doi:10.1186/1477-7827-4-5
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