Table 2

Epithelial cells express TLR3 and hormone receptor mRNA.
Cell Line
 TLR3
 ERα
 ERβ
 PRA
 PRB
AN3 CA
 -
 +
 +
 +
 +
HEC-1-A
 +
 -
 +
 -
 -
KLE
 +
 +
 +
 +
 +
MCF7
 +
 +
 +
 +
 +
RL95-2
 +
 +
 +
 +
 +

cDNA synthesized from 100 ng RNA was subjected to end-point PCR with the indicated primer sets (see Table 1). The endometrial epithelial cell lines, AN3-CA, HEC-1-A, KLE, and RL95-2 were scored for hormone receptor expression on the basis of a visible band at the appropriate size. The breast adenocarcinoma cell line, MCF7, served as a positive control for hormone receptor expression. Cell lines were compared to the RL95-2 cells for expression of TLR3.

Lesmeister et al. Reproductive Biology and Endocrinology 2005 3:74   doi:10.1186/1477-7827-3-74