Table 2

Measurement of biomarkers of oxidative stress in pregnancy

Study

Biomarkers

Methodology

Measurement units


Jauniaux et al [59]

Immunohistochemistry

Heat shock protein 70, hydroxynenal, nitrotyrosine residues.

Fluorescence intensity

Wang et al, Djordjevic et al [60, 61]

Antioxidant enzyme activity assays

Total SOD activity, catalase activity, glutathione peroxidase activity, reduced glutathione assay

Change in optical density/minutes/mg protein

Wiktor et al [62]

Oxidative DNA adducts

8-hydroxy-2 deoxyguanosine

Micromoles/mole of 2-deoxy guanosine

Holthe et al [63]

Superoxide anion, hydrogen peroxide and peroxynitrite

Dihydrethidium probe Dichlorodihydrofluorescein Dihydrorhodamine 123 Spectrophotometry/flow cytometry

Nanomoles/10 min/106 cells

Ishihara et al [64]

Lipid peroxidation products

Isoprostane, Urinary 8-epi-prostaglandin F2lpha, assayed by gas chromatography/mass spectrophotometer analysis

pg/mg of creatinine

Vaisanen-Tommiska et al [65]

Nitric oxide; Greiss reaction

Stable end products: nitrite/nitrate

Fluorometric assay, results expressed as NOx (sum of converted nitrite and very small amount of nitrite in serum).

Buhimishi et al [66]

Plasma and red blood cell glutathione content

Colorimetric assay

Nanomoles/mgm of haemoglobin


Agarwal et al. Reproductive Biology and Endocrinology 2005 3:28   doi:10.1186/1477-7827-3-28

Open Data