Figure 1.

Principle of a differential display. Test and control mRNA are separately reverse transcribed in the presence of anchored oligo(dT) primers containing nucleotides N and M in various combinations (see the text). The same primer and an arbitrary decamer are then used as primers in a PCR. The products are subjected to electrophoresis (PAGE). An additional band (see arrow) in the test sample represents a gene that had not been activated in the control sample. A11 and T11 denote eleven A and T molecules, respectively.