Figure 4.

Induction of iNOS mRNA expression in human fetal membranes (FM), cultured without serum, following treatment with LPS (1 μg/ml) alone or in combination with sodium butyrate (SB) (2 mM). Lane 1 FM control; lane 2 FM + LPS; lane 3 FM LPS + SB; lane 4 CHOiNOS positive control; lane 5 iNOS short standard; lane 6 PCR blank (no template); lane M ΦX174 DNA/Hae III Marker (Promega). Lanes 2–4 show characteristic iNOS bands at 310 base pairs (bp). One example is shown of four similar results with four different samples of fetal membranes