Table 1

Maturation-inducing and steroidogenic activities in active fractions derived from a combination of chromatofocusing followed by HICa


pg per well
 X 100


Sample
 GVBD (%)
 E2

 T
 DHP
 T/E2
 DHP/E2
FPEb
 38 ± 7
 5,850 ± 2,000
 264 ± 48
 332 ± 68
 4.5
 5.7
FPEc
 40 ± 5
 4,450 ± 2,000
 240 ± 56
 312 ± 48
 5.4
 7.0
I-1d
 12 ± 12
 3,150 ± 1,204
 147 ± 53
 126 ± 18
 4.7
 4.0
II-8d
 29 ± 8
 3,300 ± 450
 145 ± 14
 139 ± 49
 4.4
 4.2
III-10d
 4 ± 3
 2,120 ± 701
 60 ± 60
 43 ± 15
 2.8
 2.0
V-19d
 26 ± 15
 4,483 ± 393
 271 ± 23
 339 ± 91
 6.0
 7.6

a Results presented as means ± SEM derived from three chromatographic series. In the absence of added gonadotropin, no GVBD took place and T- and DHP-production were undetectable, while E2-production averaged 1,150 pg per well. The latter baseline value was subtracted from the induced E2 data to provide the indicated values for E2. b Thawed, immediately added to L-15 medium, and tested for activity at a concentration of 0.5 pit. equiv./well (0.25 pit. equiv./ml). c Thawed, stored for up to a week at 4°C, dialyzed overnight against distilled water, dried by vacuum centrifugation, reconstituted with L-15 medium, and tested for activity at a concentration of 0.5 pit. equiv./well. d Roman numerals refer to portions of the chromatofocusing runs (Fig. 4) while Arabic numerals refer to active fractions found after subsequent HIC (Fig. 5).

Lin et al. Reproductive Biology and Endocrinology 2004 2:14   doi:10.1186/1477-7827-2-14