Figure 5.

Mono-P fractions #I-V (containing Polybuffer) were readjusted with start buffer [1.2 M (NH₄)₂SO₄ in 0.1 M sodium phosphate, pH 6.8]. Each individual Mono-P fraction was rechromatographed on a Phenyl-5-PW column. A linear gradient of decreasing salt concentration was started after the Polybuffer eluted. Shaded areas indicate fractions containing gonadotropic activities (see Table 1).