Figure 1.

Size-exclusion chromatography (SEC) of FPE. Approximately 78 pituitary equivalents in a volume of 200 μl were applied to the Superose columns and the effluent was monitored for UV-absorbance (A). One-ml fractions were collected and tested for (B) maturational and (C) steroidogenic activity. For this and subsequent figures, negative controls (no gonadotropic extracts or fractions added) had 0–5% GVBD, DHP and T secretion were not detectable, and E₂ secretion averaged less than 0.5 ng/2-ml well. For this experiment only, positive controls (FPE added at a concentration of 0.5 pit. equiv./well) were 65% GVBD with DHP-, T-, and E₂-secretion of 1.25, 0.75, and 7.4 ng/well, respectively.