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Open Access Research

Efficient induction of spawning of Northern leopard frogs (Lithobates pipiens) during and outside the natural breeding season

Vance L Trudeau*, Frederick W Schueler, Laia Navarro-Martin, Christine K Hamilton, Elizabeth Bulaeva, Amanda Bennett, William Fletcher and Lisa Taylor

Reproductive Biology and Endocrinology 2013, 11:14  doi:10.1186/1477-7827-11-14

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CORRECTION TO PROTOCOL FROM SENIOR AUTHOR

Vance Trudeau   (2014-03-14 11:07)  University of Ottawa email

Hello, a colleague noted a typographical error that relates to the dose of GnRH-A. I would like to correct that here.  Go to  section 2 (one page 3) of the methods in paragraph 3, there is a symbol error.

The original sentence is:

"For the priming pre-treatment, females and males were injected i.p. between 14:00- 14:30 with a low dose of GnRH-A (0.04 mg/g; Day 1)".

THE CORRECTED SENTENCE IS :

For the priming pre-treatment, females and males were injected i.p. between 14:00- 14:30 with a low dose of GnRH-A (0.04 MICROGRAMS/g; Day 1).

TO BE CLEAR: Please note that the dose of GnRH-A for priming is 0.04 micrograms per gram body weight not 0.04 millgrams per gram.  It is clear that the "micro" symbol is missing.The principle behind this step is to give a lower dose of GnRH-A than that is in the AMPHIPLEX mix.  In this way,  one can sensitize the pituitary.  This is the concept of GnRH priming described in the paper.

I am sorry about this oversight.  If you ahve any further questions, please do not hesitate to contact me. Good luck with your experiments. Yours Sincerely, Vance Trudeau (trudeauv@uottawa.ca).

Competing interests

None,  I am the author.

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