Figure 1.

Confocal laser scanning micrographs of fully-grown mouse germinal vesicle intact oocytes double labeled with ABL₂ and LCA. (A) LCA and (B) ABL₂ labeled the cortical granules of a germinal vesicle intact oocyte. (C) Merged images of both ABL₂ and LCA labeled germinal vesicle intact oocytes showed two populations of cortical granules (red and yellow). (D) Three-dimensional reconstruction of LCA and ABL₂ labeled oocytes showing the two populations of cortical granules. Control oocytes were not labeled by Texas Red-streptavidin (E) or goat anti-rabbit IgG conjugated to Alexa 488 (F) alone.