Figure 4.

Challenge of cumulus cell monolayers with conditioned media from pig oocytes cultured in vitro under different, unfavorable conditions, namely COCs without hormonal support (M-H), and matured denuded oocytes (MD), compared to immature (GV) and properly matured cells (M+H). Histograms represent the mean E₂ production ± sd. Control wells were treated as in fig 2 and 3. * P < 0.01 (Student t-test).