Figure 1.

Immunohistochemical staining of cytospin preparations of HEC295T cells transfected or not with DNA constructs expressing either LGR7, LGR8 or the orphan G-protein coupled receptor, HE6. Each panel is annotated to indicate, from left to right, the epitope against which the antibody has been raised (e.g. L7-2), then the animal used for this epitope (e.g. R3), and finally the expression construct transiently transfected into the cells (e.g. LGR7, LGR8 or HE6). All antisera against epitopes L7-1 and L7-2 were diluted 1:4000: those against L7-3 were diluted 1:5000. Arrowheads point to positively immunostained cells. Negative controls are shown in panels B, C, G, H, M and N. Positive controls for the LGR8 and HE6 transfections are shown in panels H and O, respectively. Scale bar = 50 μm.